The Examination of Antigens in Highly Purified Immune Complexes
- 1 January 1979
- journal article
- research article
- Published by Taylor & Francis in Immunological Communications
- Vol. 8 (5-6), 545-553
- https://doi.org/10.3109/08820137909063253
Abstract
A combination of gel filtration and affinity chromatography was used for preparation of immune complexes (IC) from sera of individuals with systemic lupus erythematosus (SLE) and others with no apparent disease. Overlaying of gels with patient or normal sera, counterstaining with 125I-protein A and autoradiography showed besides immunoglobulins, the presence of 6 unidentified antigens with molecular weights below 42,000 dalton. They were not exclusively associated with SLE IC.This publication has 18 references indexed in Scilit:
- Analysis of the components of immune complexesMolecular Immunology, 1979
- Isolation of circulating immune complexes using Raji cells. Separation of antigens from immune complexes and production of antiserum.JCI Insight, 1978
- Isolation of soluble immune complexes by affinity chromatography using staphylogoccal protein A-Sepharose as substrateJournal of Immunological Methods, 1977
- Immune complexes in hepatitis B antigen-associated periarteritis nodosaAmerican Journal Of Medicine, 1977
- The Raji cell radioimmune assay for detecting immune complexes in human sera.JCI Insight, 1976
- Protein A Reactivity of Two Distinct Groups of Human Monoclonal IgMScandinavian Journal of Immunology, 1975
- The pathogenesis of arthritis associated with acute hepatitis-B surface antigen-positive hepatitis. Complement activation and characterization of circulating immune complexes.JCI Insight, 1975
- The measurement of IgG anti-IgG antibody in rheumatoid arthritisJournal of Immunological Methods, 1974
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970
- Antiglobulin factors in sera from patients with rheumatoid arthritis and normal subjects. Quantitative estimation in different immunoglobulin classes.Annals Of The Rheumatic Diseases, 1967