Rat medulla oblongata. IV. Topographical distribution of catecholaminergic neurons with quantitative three‐dimensional computer reconstruction
- 15 March 1985
- journal article
- research article
- Published by Wiley in Journal of Comparative Neurology
- Vol. 233 (3), 350-364
- https://doi.org/10.1002/cne.902330305
Abstract
We examined serial 40 μm vibratome, immunoperoxidase-stained sections of the medulla with tyrosine hydroxylase (TH), dopamine-beta-hydroxylase (DBH), and phenylethanolamine N-methyltransferase (CPNMT) antisera followed by Nissl staining to locate catecholaminergic neurons in cytoarchitectonic regions followed by a three-dimensional (3D) computer reconstruction of these cell groups to determine their spatial organization. Overlay drawings of low and high power photomicrographs showing cell bodies and nuclear boundaries were entered into a digital computer storage system. Every section in the series was plotted to yield an accurate representation of regional densities of cells and location of nuclei, as revealed by two-dimensional plots of individual sections as well as three-dimensional plots of groups of sections. Data files were scanned in a number of ways to obtain total cell counts of TH-, DBH-, and PNMT-immunoreactive cells within a designated area or cell counts of only one type of immunoreactive cell. This combination of data manipulation produced the following results: (1) A1 group is a homogeneous population of noradrenergic neurons at levels caudal to the obex, and at the obex it is mixed with adrenergic cells. The dimensions of the A1 cell group are 1.3 × 2.7 mm, extending from −2.5 to +0.2. Part of this cell group lies in the lateral reticular nucleus. (2) A2 group is not purely noradrenergic as previously suspected. It is a very mixed cell group containing mainly dopaminergic neurons in the area postrema (periventricular region) and the dorsal motor nucleus of the vagus, mainly noradrenergic neurons in the medial subnucleus of the nucleus of the tractus solitarius (nTS), mainly adrenergic neurons in the dorsal strip and dorsal subnucleus of the nucleus of the tractus solitarius, and a mixture of all three catecholaminergic neurons in the other subnuclei of the nTS. The dimensions of this group are 0.4 × 3 mm extending from −2.7 to +0.3. (3) C1 group is a homogeneous population of adrenaline cells extending from +1 to +2.5 with dimensions of 1.5 × 1.5 mm and consisting of scattered neurons some of which occupy the gigantocellular reticular nucleus. (4) C2 group is a homogeneous population of adrenaline neurons extending from +1 to +3 with dimensions of 2.5 × 3 mm. Accurate visual imaging and quantitation of the spatial organization of medullary catecholaminergic neurons within the classical anatomical framework of cytoarchitecture provides an enhanced comprehension of the organization of this region of the central nervous system.Keywords
This publication has 10 references indexed in Scilit:
- Rat medulla oblongata. II. Dopaminergic, noradrenergic (A1 and A2) and adrenergic neurons, nerve fibers, and presumptive terminal processesJournal of Comparative Neurology, 1985
- Rat medulla oblongata. III. Adrenergic (C1 and C2) neurons, nerve fibers and presumptive terminal processesJournal of Comparative Neurology, 1985
- Distribution of neuropeptide immunoreactive nerve terminals within the subnuclei of the nucleus of the tractus solitarius of the ratJournal of Comparative Neurology, 1984
- A general system for computer based acquisition, analysis and display of medical image dataPublished by Association for Computing Machinery (ACM) ,1982
- Brain stem projections of sensory and motor components of the vagus complex in the cat: II. Laryngeal, tracheobronchial, pulmonary, cardiac, and gastrointestinal branchesJournal of Comparative Neurology, 1980
- Brain stem projections of the aortic nerve in the cat: A study using tetramethyl benzidine as the substrate for horseradish peroxidaseBrain Research, 1980
- Immunohistochemical evidence for the existence of adrenaline neurons in the rat brainBrain Research, 1974
- Evidence for Adrenaline Neurons in the Rat BrainActa Physiologica Scandinavica, 1973
- Enzymes Involved in the Catalysis of Catecholamine BiosynthesisPublished by Springer Nature ,1972
- FUNCTIONAL LOCALIZATION OF CENTRAL COORDINATING MECHANISM FOR EMESIS IN CATJournal of Neurophysiology, 1949