Chromatographic Studies on Leucine Aminopeptidase in Normal Human Granulocytes and in Cells of Acute and Chronic Myeloid Leukemia

Abstract

The LAP activity in leukocytes of acute myeloblastic leukemia was lower than in normal granulocytes. The activity tends to be higher than normal in chronic myeloid forms. Chromatographic fractionation using DEAE-cellulose of lysates of normal granulocytes revealed many protein fractions with LAP activity. The non-adsorbed fraction and the first fraction absorbed by the column appear to be absent in the chromatograms obtained from leukolysates of acute leukemia. These fractions are present, although remaining moderate in size, in the phase of remission of the disease. On the other hand, in the case of chronic leukemia the chromatographic pattern presents particularly marked peaks in the first fractions.