Alkaline O→N-transacylation. A new method for the quantitative deacylation of phospholipids
- 1 April 1981
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 195 (1), 301-306
- https://doi.org/10.1042/bj1950301
Abstract
1. Quantitative O-deacylation of phospholipids has been achieved by incubation with a reagent containing monomethylamine, methanol and water. The reaction is primarily an O leads to N-transacylation with N-methyl fatty acid amides being formed. 2. The reagent can be removed easily by volatilization and under defined conditions no secondary decomposition of the phosphorus-containing deacylation products occurs. 3. The water-soluble phosphorus compounds derived by deacylation of mammalian tissue O-diacylated phospholipids have been completely separated by a single-dimensional paper ionophoresis with a volatile pH9 buffer. 4. The O-deacylated alkyl and alkenyl phospholipids have been examined by t.l.c. before and after catalytic hydrolysis with Hg2+. 5. A complete analysis of rat brain phospholipids by the above methods agrees closely with that obtained by other procedures.This publication has 16 references indexed in Scilit:
- The selective loss of lysophospholipids in some commonly used lipid-extraction proceduresAnalytical Biochemistry, 1974
- Modified spray for the detection of phospholipids on thin-layer chromatogramsJournal of Lipid Research, 1968
- Two-dimensional chromatography on silica gel-loaded paper for the microanalysis of polar lipidsJournal of Lipid Research, 1966
- SIMPLIFIED PREPARATION OF L-α-GLYCERYL PHOSPHORYL CHOLINECanadian Journal of Biochemistry, 1965
- INCORPORATION OF ORTHO [32P]PHOSPHATE INTO THE SUBCELLULAR FRACTIONS OF DEVELOPING RAT BRAIN*Journal of Neurochemistry, 1965
- Breakdown of phospholipids in mild alkaline hydrolysisJournal of Lipid Research, 1963
- Improvements in the method of determining individual phospholipids in a complex mixture by successive chemical hydrolysesBiochemical Journal, 1962
- The isolation of an alpha'-alkoxy-beta-acyl-alpha-glycerophosphorylethanolamine from bovine erythrocytes.1961
- Reaction of Mercuric Chloride with PlasmalogenNature, 1959
- Cyclic Glycerophosphate Formation from the GlycerolphosphatidesJournal of Biological Chemistry, 1959