A single gel analysis of 575 dominant and codominant restriction landmark genomic scanning loci in mice interspecific backcross progeny

Abstract

We identified 575 new NotI landmarks of C57BL/6(B)‐ and, M. spretus (S)‐specific, dominant and codominant loci which were segregated in B × S interspecific backcrosses (BSS), using the restriction landmark genomic scanning (RLGS) spot mapping method. All of these loci were visualized on a single RLGS profile which was produced with NotI‐PvuII‐PstI. These landmarks include 250 newly identified S‐specific spots in addition to the previously reported 325 B‐specific spots. The S‐specific spots were identified by reading full or half intensity, based on the property that the spot intensity of the autoradiographic signal reflected the copy number of an end‐labeled restriction landmark. The cumulative map is 1341 cM and it is based upon 985 meiotic events in 72 backcross progeny. This map covers 90% of the total estimated length of the mouse genetic map. This map provides a good tool for the high‐speed genome scanning assay in the mouse genome by a single RLGS gel analysis.