Differential distribution of endothelin peptides and receptors in human adrenal gland
- 1 November 1996
- journal article
- Published by Springer Nature in Journal of Molecular Histology
- Vol. 28 (11), 779-789
- https://doi.org/10.1007/bf02272151
Abstract
Sub-type selective ligands revealed a differential distribution of endothelin (ET) receptors within human adrenal glands. High densities of ETA receptors were localized, using [125I]-PD151242, to the smooth muscle layer of the arteries, smaller vessels within the capsular plexus and to the secretory cells of zona glomerulosa (K D=139.8±39.7,B max=69.7±9.1 fmol mg−1 protein, mean of 3 individuals±sem). ETB receptors were present in the medulla (K D=145.2±16.4,B max=75.5±12.3), zona glomerulosa (KD=100.6±35.1,B max=63.1±10.0), fasiculata (K D 145.1±162.,B max=67.9±6.9) and reticularis (KD=118.2±18.6,B max=71.9±6.5). ETB receptors were not detected within the smooth muscle of the vasculature. Messenger RNA encoding both sub-types was present in adrenals. ET-like immunoreactivity was localized to the cytoplasm of the endothelial cells from arteries supplying the gland and resistance vessels within the capsular plexus. Staining was also detected in these cells using anti-big ET-1 and less intensely with anti-big ET-2 antisera but not within cells within the cortex or medulla. Big ET-3-like immunoreactivity was localized to secretory cells of the medulla. Staining was not found using antiserum that could detect ET-3, suggesting further processing of big ET-3 may occur within the plasma, and that the cdrenals could be a source of ET-3. The presence of ET-1 was confirmed by high performance liquid chromatography and radioimmunoassay although ET-3 was not detected. The results suggest that ET-1 is the predominant mature isoform, which is localized mainly to adrenal vasculature, particularly the capsular plexus, and may contribute to blood flow regulation in the gland.Keywords
This publication has 36 references indexed in Scilit:
- Autoradiographic Study of Endothelin Receptors in Human Cerebral ArteriesJournal of Cardiovascular Pharmacology, 1995
- Gene expression, localization, and characterization of endothelin A and B receptors in the human adrenal cortex.JCI Insight, 1994
- Is endothelin-induced vasoconstriction mediated only by ETA receptors in humans?Trends in Pharmacological Sciences, 1994
- Human Kidney: Endothelin Isoforms Detected by HPLC with Radioimmunoassay and Receptor Subtypes Detected Using Ligands BQ123 and BQ3020Journal of Cardiovascular Pharmacology, 1993
- Concomitant Expression of Endothelin Receptor Subtype and Isopeptide by Pheochromocytoma and Bovine Adrenal Medulla.Hypertension Research, 1993
- Anatomical localization and pharmacological activity of mature endothelins and their precursors in human vascular tissueJournal Of Hypertension, 1992
- Concomitant expression of receptor subtype and isopeptide of endothelin by human adrenal glandBiochemical and Biophysical Research Communications, 1992
- Regional Hemodynamic Effects and Clearance of Endothelin-1 in HumansJournal of Cardiovascular Pharmacology, 1992
- Cloning and chromosomal localization of a human endothelin ETA receptorBiochemical and Biophysical Research Communications, 1991
- Endothelin-3 concentrations in human plasma: The increased concentrations in patients undergoing haemodialysisBiochemical and Biophysical Research Communications, 1990