Antigenic Proteins of Nucleolar Chromatin of Novikoff Hepatoma Ascites Cells
Open Access
- 1 July 1977
- journal article
- research article
- Published by SAGE Publications in Tumori Journal
- Vol. 63 (4), 347-357
- https://doi.org/10.1177/030089167706300406
Abstract
Nucleolar chromatin of Novikoff hepatoma ascites cells contains an antigen (no-Ag1) detected with antinucleolar antibodies by the immunodiffusion technique. This antigen was distinguished from the previously reported nuclear chromatin antigen NAg-1 (19) by the findings that tumor nucleolar antibodies which formed immunoprecipitin bands with no-Ag1 did not do so with NAg-1 and that tumor cytosol, which contains NAg-1, formed immunoprecipitin bands with tumor chromatin antibodies but not with antibodies to tumor nucleoli. Tumor nucleolar chromatin contains both NAg-1 and no-Ag1, but only no-Ag1 formed bands with tumor nucleolar antibodies. no-Ag1 is a component of tumor nucleolar chromatin that was not soluble in 0.075 M NaCl - 0.025 M EDTA, pH 8, and only slightly soluble in 0.01 M Tris-HCl, pH 8. no-Ag1 was not found in liver nucleoli. Antibodies to liver nucleoli formed immunoprecipitin bands with liver nucleolar antigens but none were confluent with those formed between tumor nucleolar antibodies and antigens of tumor nucleolar chromatin. Absorption of the tumor nucleolar antibodies with whole tumor cells or whole liver pressate did not alter band formation with no-Ag1. Three antigens in liver nucleoli were not found in tumor nucleoli.This publication has 13 references indexed in Scilit:
- A fetal protein in chromatin of Novikoff hepatoma and Walker 256 carcinosarcoma tumors that is absent from normal and regenerating rat liver.Proceedings of the National Academy of Sciences, 1976
- Role of ribosomal RNA methylases in the regulation of ribosome production in mammalian cellsBiochemistry, 1976
- Effect of extradiol-17beta on the synthesis of specific uterine nonhistone chromosomal proteins.Proceedings of the National Academy of Sciences, 1975
- Nuclear phosphoprotein kinase activities in normal and neoplastic tissuesBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1975
- Extractable nuclear antigen effect on the DNA anti-DNA reaction and NZB/NZW mouse nephritis.JCI Insight, 1975
- Chapter 18 The Isolation of Nuclei with Citric Acid and the Analysis of Proteins by Two-Dimensional Polyacrylamide Gel ElectrophoresisMethods in Cell Biology, 1975
- Evaluation of epstein‐barr virus‐associated nuclear antigen with various human cell linesInternational Journal of Cancer, 1974
- Le récepteur de l'oestradiol, l'AMP cyclique et la RNA polymérase nucléolaire dans l'utérus de génisse: Stimulation de la biosynthèse de RNA in vitroBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1971
- Antigenic and cultural properties of cells doubly transformed by polyoma virus and SV40Virology, 1965
- SPECIFIC CARCINOEMBRYONIC ANTIGENS OF THE HUMAN DIGESTIVE SYSTEMThe Journal of Experimental Medicine, 1965