A combined flat‐embedding, HRP histochemical method for correlative light and electron microscopic study of single neurons
- 1 January 1984
- journal article
- research article
- Published by Wiley in Journal of Neuroscience Research
- Vol. 11 (1), 27-34
- https://doi.org/10.1002/jnr.490110104
Abstract
A simple and reproducible method is described for studying the morphology of the same neuron at the light and electron microscopic level. This method utilizes horseradish peroxidase histochemistry and a recently described flat-embedding procedure wherein thin, aldehyde-fixed sections are placed in resin between glass microscope slides pretreated with dimethyldichlorosilane. The significance of these combined methodologies for correlative light and electron microscopic studies of single neurons is discussed.Keywords
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