STUDIES OF BUDDING AND CELL WALL STRUCTURE OF YEAST

Abstract
Thin sections of yeast cells and "purified" yeast cell walls have been prepared and examined with the electron microscope. Some of the stages of the budding process have been described. The yeast cell wall consists of several distinct layers. Observations of cell wall structure made on sections of whole cells and isolated cell walls were in general agreement, with the exception that an outer layer of electron dense material was consistently present in whole cells but not in isolated cell walls. Details of the internal structure of the cells were obscured due to difficulties of fixation and sectioning.

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