The Structural Conservation of S100 Protein During Evolution: Analysis by Reactivity with a Monoclonal Antibody
- 1 January 1983
- journal article
- research article
- Published by Wiley in Journal of Neurochemistry
- Vol. 41 (1), 107-112
- https://doi.org/10.1111/j.1471-4159.1983.tb11820.x
Abstract
A hybridoma cell line producing a monoclonal antibody (A4) against bovine S100 protein was produced by fusing mouse myeloma P3X63/Ag8 cells with spleen cells from a BALB/c mouse immunized with bovine S100 protein. A4 is of the IgG2b subclass and was purified by affinity chromatography on a protein A-Sepharose column. Brain extracts from several mammalian and 1 avian species reacted both with polyclonal rabbit anti-S100 protein antiserum and with A4 in a radioimmunoassay. Brain extract from dog was a notable exception. It reacted with the rabbit antiserum but not with A4. A4 reacts with a common epitope that is present on S100 proteins from different vertebrae species but is absent on dog S100 protein.Keywords
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