Rapid mtDNA Sequence Analysis of Fish Populations Using the Polymerase Chain Reaction (PCR)
- 19 December 1991
- journal article
- Published by Canadian Science Publishing in Canadian Journal of Fisheries and Aquatic Sciences
- Vol. 48 (S1), 95-98
- https://doi.org/10.1139/f91-307
Abstract
The polymerase chain reaction (PCR) is a relatively new technique allowing the enzymatic amplification of selected genes to produce quantities sufficient for the determination of nucleotide sequences. We have compared sequences of several genes from the mitochondrial genome of six salmonid species. PCR analysis of protein coding genes provides sufficient resolution for determining relationships among species, or among isolated populations. Examination of more variable regions, such as the control region of the mitochondrial genome, will be required for other intraspecific comparisons.Keywords
This publication has 6 references indexed in Scilit:
- Variation in salmonid mitochondrial DNA: Evolutioinary constraints and mechanisms of substitutionJournal of Molecular Evolution, 1989
- Dynamics of mitochondrial DNA evolution in animals: amplification and sequencing with conserved primers.Proceedings of the National Academy of Sciences, 1989
- Primer-directed enzymatic amplification of DNA with a thermostable DNA polymeraseScience, 1988
- Mitochondrial DNA analysis of Pacific salmonid evolutionCanadian Journal of Zoology, 1986
- Colony probing as an alternative to standard sequencing as a means of direct analysis of chromosomal DNA to determine the spectrum of single-base changes in regions of known sequence.Proceedings of the National Academy of Sciences, 1986
- Specific Enzymatic Amplification of DNA In Vitro: The Polymerase Chain ReactionCold Spring Harbor Symposia on Quantitative Biology, 1986