Maize flour, potato flour, cocoyam flour, plantain flour, yam flour, and rice flour were assayed for starch digestibility by an established enzymatic procedure. These were either autoclaved, microwaved, or parboiled and then freeze-dried. Freeze-dried samples were stored for 10 days either below freezing or at ambient temperature. Parameters assessed were readily digestible starch (RDS), slowly digestible starch (SDS), and total starch (TS). Data was analyzed by t-test (P < or = 0.05). RDS levels among raw flours ranged from 1.01 g/100g in rice flour to 8.16 g/100 g in cocoyam flour. Autoclaving and parboiling increased RDS levels in most flours, while microwaving significantly reduced RDS compared with raw flour. Ambient temperature storage reduced the RDS content. SDS levels ranged from 4.95 g/100 g in yam flour to 22.2 g/100 g in maize flour. SDS levels were increased by autoclaving and parboling, but significantly reduced by microwaving, compared with the raw flour. Storage at ambient temperature resulted in lower SDS. The TS content in raw flour ranged from 28.0 g/100 g in plantain flour to 68.4 g/100 g in rice flour. Autoclaving resulted in reduced TS levels insome flours. Moist heat processing and the post-process storage temperature therefore result in significant changes in starch susceptibility to enzymic digestion. This information will be useful in developing food processing and storage procedures that modify starch resistance to digestion in order to optimize its nutritional quality and to enhance the physiological benefits.