Spontaneous cerebral hypothermia diminishes focal infarction in rat brain.
- 1 December 1992
- journal article
- abstracts
- Published by Wolters Kluwer Health in Stroke
- Vol. 23 (12), 1812-1816
- https://doi.org/10.1161/01.str.23.12.1812
Abstract
Brain temperature during ischemia is known to strongly influence the extent of cellular injury. The objectives of the present study were to determine the effect of severe focal ischemia on brain temperature and to assess the influence of those changes on focal infarction. Severe focal ischemia was produced in rats using permanent occlusion of the distal middle cerebral artery combined with transient (60-minute) bilateral carotid artery occlusion. The temperature of the ischemic focus was measured with a small subdural probe. Three groups of rats were studied. In the first group, brain temperature was permitted to decline spontaneously to 32 degrees C after occlusion. In the second, brain temperature was maintained at 37.5 degrees C during occlusion. In the third group, the brain temperature was maintained at 37.5 degrees C for 40 minutes postocclusion before cooling. After recovery for 24 hours, the volume of infarction was measured in histological sections. In the absence of cranial heating, the brain temperature fell to 33 degrees C by 10 minutes postocclusion, and infarct volume was 19 +/- 9 mm3 (mean +/- SEM; n = 6). Maintaining brain temperature at 37.5 degrees C increased the volume of infarction to 82 +/- 16 mm3 (n = 7; p < 0.001). Delayed cooling did not prevent the increase in infarct volume (75 +/- 16 mm3; n = 6). These results demonstrate that in the present model of transient focal ischemia, spontaneous cooling of the brain during ischemia diminishes the extent of focal infarction, relative to that observed when cerebral hypothermia is prevented or delayed for 40 minutes.Keywords
This publication has 17 references indexed in Scilit:
- Regional Expression of Heat Shock Protein-70 mRNA and c-Fos mRNA following Focal Ischemia in Rat BrainJournal of Cerebral Blood Flow & Metabolism, 1992
- Transient Hypothermia Reduces Focal Ischemic Brain Injury in the RatNeurosurgery, 1991
- Postischemic Hypothermia Fails to Reduce Ischemic Injury in Gerbil HippocampusJournal of Cerebral Blood Flow & Metabolism, 1991
- Mild Hypothermia Prevents Ischemic Injury in Gerbil HippocampusJournal of Cerebral Blood Flow & Metabolism, 1990
- The Influence of Mild Body and Brain Hypothermia on Ischemic Brain DamageJournal of Cerebral Blood Flow & Metabolism, 1990
- The evolution of focal ischemic damage: A metabolic analysisMetabolic Brain Disease, 1990
- Postischemic moderate hypothermia inhibits CA1 hippocampal ischemic neuronal injuryNeuroscience Letters, 1989
- Focal Brain Ischemia in the Rat: Methods for Reproducible Neocortical Infarction Using Tandem Occlusion of the Distal Middle Cerebral and Ipsilateral Common Carotid ArteriesJournal of Cerebral Blood Flow & Metabolism, 1988
- Small Differences in Intraischemic Brain Temperature Critically Determine the Extent of Ischemic Neuronal InjuryJournal of Cerebral Blood Flow & Metabolism, 1987
- Focal Cerebral Ischaemia in the Rat: 1. Description of Technique and Early Neuropathological Consequences following Middle Cerebral Artery OcclusionJournal of Cerebral Blood Flow & Metabolism, 1981