The genus Leishmania.

Abstract

The systematic position of the so-called "species" of Leishmania is examined and an attempt made to determine their phylogenetic relationships. The morphology of the organisms as seen by light- and electron-microscopy is described; neither method provides useful criteria for the determination of species. The behaviour of the parasites in insect and in vertebrate hosts offers a better method of classification. In this way, the species may be divided into 4 main groups, comprising the mammalian species involving man, the distinctive species L. enriettii in the guinea-pig, those infecting lizards, and species apparently in various stages of evolution in phlebotomines. The so-called "human" group is divided into visceral forms (originating chiefly in wild canidae) and cutaneous forms (probably of rodent origin). The named species of the former group include L. donovani and L. infantum. The cutaneous species include L. tropica tropica (=minor), L. tropica major, L. brasiliensis, L. peruana, L. guyanensis, and L. mexicana. L. pifanoi is probably not a distinct species but represents various forms as modified by the failure of cell-mediated immunity in the host. Leishmanial infections can be identified first by ascertaining the geographical area where the infection was acquired, and then by more or less complicated laboratory investigations including characteristics in culture, serological tests, the response of special hosts in terms of symptomatology, and the behaviour of the parasite in the phlebotomine host. No test is infallible, and an effective simple test is urgently needed. The preservation of Leishmania strains is an important research procedure and a method for conserving parasites by lyophilization is described briefly.