Formation of hybridoma clones in soft agarose: Effect of pH and of medium
- 1 May 1980
- journal article
- research article
- Published by Springer Nature in Somatic Cell and Molecular Genetics
- Vol. 6 (3), 435-441
- https://doi.org/10.1007/bf01542794
Abstract
Optimal conditions for the formation of hybridoma clones in soft agarose are described. The hybridization frequency is shown to be highly dependent on the pH of the polyethylene glycol (PEG) solution used for fusion and on the cloning medium. Maximal numbers of clones are obtained when the PEG solution used for fusion is at pH 8.0–8.2.Keywords
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