XenopusNF-Y pre-sets chromatin to potentiate p300 and acetylation-responsive transcription from theXenopus hsp70promoterin vivo

Abstract

We identify Xenopus NF‐Y as a key regulator of acetylation responsiveness for the Xenopus hsp70 promoter within chromatin assembled in Xenopus oocyte nuclei. Y‐box sequences are required for the assembly of DNase I‐hypersensitive sites in the hsp70 promoter, and for transcriptional activation both by inhibitors of histone deacetylase and by the p300 acetyltransferase. The viral oncoprotein E1A interferes with both of these activation steps. We clone Xenopus NF‐YA, NF‐YB and NF‐YC and establish that NF‐Y is the predominant Y‐box‐binding protein in Xenopus oocyte nuclei. NF‐Y interacts with p300 in vivo and is itself a target for acetylation by p300. Transcription from the hsp70 promoter in chromatin can be enhanced further by heat shock factor. We suggest two steps in chromatin modification at the Xenopus hsp70 promoter: first the binding of NF‐Y to the Y‐boxes to pre‐set chromatin and second the recruitment of p300 to modulate transcriptional activity.