Nutrition of Animal Cells in Tissue Culture. II. Use of Tweens in Synthetic Feeding Mixtures.
- 1 May 1950
- journal article
- research article
- Published by Frontiers Media SA in Experimental Biology and Medicine
- Vol. 74 (1), 22-26
- https://doi.org/10.3181/00379727-74-17797
Abstract
The effect of Tweens 20, 40, 60, 80 and 85, and of oleic acid, upon the survival of chick embryo mesenchyme cells maintained in a completely synthetic feeding mixture was studied over the concn. range of 0.5% to 0.00005%. Levels of 0.5% and 0.05% were definitely toxic whereas lower concns. were not. Relatively small amts. of ethyl alcohol (0.3%) were inhibitory when used together with low concns. of Tweens. Reduction of the alcohol content to a min. (0.01%) makes it possible to employ the Tweens as a source of water-soluble fatty acids and also to dissolve fat-soluble compounds in aqueous synthetic feeding mixtures.Keywords
This publication has 4 references indexed in Scilit:
- Nutrition of Animal Cells in Tissue Culture. I. Initial Studies on a Synthetic Medium.,Experimental Biology and Medicine, 1950
- SUGAR ALCOHOLS .26. PHARMACODYNAMIC STUDIES OF POLYOXYALKYLENE DERIVATIVES OF HEXITOL ANHYDRIDE PARTIAL FATTY ACID ESTERS1948
- OLEIC ACID AS A GROWTH STIMULANT FOR LACTOBACILLUS CASEIJournal of Biological Chemistry, 1946
- The effect of unsaturated fatty acids on Lactobacillus helveticus and other Gram-positive micro-organismsBiochemical Journal, 1945