COMPARATIVE EFFECTS OF IODOACETATE AND IODOACETAMIDE ON THE OXYGEN CONSUMPTION AND GLYCOLYSIS OF FROG MUSCLE

Abstract

The acetate is a much more rapid inhibitor of glycolysis of resting frog muscle, muscle mash, and muscle extract, at a given conc., than the amide. This is the reverse of the order of effectiveness known for these compounds in their reaction with compounds containing the sulfhydryl group. Although the amide inhibits respiration more rapidly than the acetate at physiological pH, both compounds are equally effective at more acid pH values. The difference at physiological pH is accounted for in part by more rapid penetration of the amide. Neither respiration nor glycolysis indicates by these criteria that the mechanism of inhibition by the iodoacetyl group involves combination with an intracellular sulfhydryl compound.