Homologous upstream sequences near Epstein-Barr virus promoters.

Abstract
The sequence of the 17,166-base-paire EcoRI C fragment of Epstein-Barr virus DNA, [human lymphoblastoid] cell line B95-8, was determined. In vitro transcription was used to identify 3 RNA polymerase II promoters within this fragment of the virus. Cytoplasmic poly(A)+RNA starting at these points were demonstrated in B95-8 cells induced into virus production with 12-O-tetradecanoylphorbol 13-acetate. Uninduced B95-8 cells contained much less of these RNA. The upstream sequences near the 3 promoters show striking homologies that may be involved in transcriptional control.