Abstract
L forms developed from group A hemolytic streptococci when they were exposed to penicillin on horse serum agar plates containing high concentrations of NaCl (0.5 [image]). Subcultures grew on a similar medium and were morphologically similar to the L forms of other bacteria. Retransfor-mation to the coccus occurred on penicillin free medium after numerous transfers in the L form. Increased concentration of other salts including KC1, NH4Cl, CaCl2, MgCl2 and Na2HPO4 also permitted isolation of the L form of the hemolytic streptococcus but less regularly than with high concentration of NaCl.

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