Tricarboxylic Acid Cycle Mutants in Saccharomyces: Comparison of Independently Derived Mutants

Abstract
A yeast mutant independently isolated as a glutamate auxotroph (glt2-1) was similar to the glt1-1 mutant in exhibiting a blocked tricarboxylic acid cycle due to the lack of aconitate hydratase. The new mutant differed by exhibiting blocks in lysine and cytochrome biosynthesis which segregated together with the glutamate requirement.