The Relation of Ribose Nucleic Acid to the Early Stages of Induced Enzyme Synthesis in Yeast

Abstract

SUMMARY: The synthesis of deoxyribonucleic acid (DNA) in yeast can be abolished by dosages of ultraviolet light (UV) which permit ribonucleic acid (RNA) and protein synthesis to continue. Those dosages of UV which inhibit α-glucosidase synthesis prevent not only the net utilization of the free amino acid pool but also inhibit glycine incorporation into proteins and decrease to a minimal value glycine and phosphate incorporation into RNA. The latent period before the appearance of α-glucosidase was characterized by an increased sensitivity to irradiation and certain amino acid analogues. The significance of these results in an interpretation of the early stages in enzyme induction is discussed.