EFFECT OF DIETHYLAMINOETHYL-DEXTRAN ON COLONY FORMATION OF HUMAN-TUMOR CELLS IN SEMISOLID SUSPENSION-CULTURES
- 1 January 1983
- journal article
- research article
- Vol. 43 (1), 210-213
Abstract
The effect of diethylaminoethyl-dextran on colony formation of established and primary human ovarian and breast tumor cells [MDA-MB231, MDA-MB435, MDA-MB436 and MDA-MB468] in semisolid suspension cultures was investigated. At the concentration of 250-300 .mu.g/ml used in the human tumor stem cell assay, diethylaminoethyl-dextran inhibited in vitro growth of 3 of 4 human breast tumor cell lines and of 10 of 19 primary tumors. It did not affect growth in 7 primary tumors and enhanced colony formation in 2 tumors. Diethylaminoethyl-dextran may interact in the human tumor stem cell assay with other sites additional to sulfuric acid residues of the aga and these effects may cancel or outweigh its purported beneficial effects.This publication has 6 references indexed in Scilit:
- Transforming growth factors produced by certain human tumor cells: polypeptides that interact with epidermal growth factor receptors.Proceedings of the National Academy of Sciences, 1980
- ARREST OF CELL-MEMBRANE MOVEMENTS BY INVITRO INCUBATION WITH POLYCATION REVERSED BY POLYANION1979
- Regression of Established Tumors in Rats by Injection of Diethylaminoethyl-Dextran and Friend Murine Leukemia Virus2JNCI Journal of the National Cancer Institute, 1978
- Incorporation of deoxynucleotides into DNA by diethylaminoethyldextran-treated lymphocytesBiochemistry, 1977
- Primary Bioassay of Human Tumor Stem CellsScience, 1977
- A Membrane Effect of Basic Polymers dependent on Molecular SizeNature, 1967