Characteristics of polyamine stimulation of cyclic nucleotide-independent protein kinase reactions
- 15 December 1985
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 232 (3), 767-771
- https://doi.org/10.1042/bj2320767
Abstract
The extent of direct stimulation by spermine of reactions catalysed by nuclear N1 and N2 protein kinases purified from liver and prostate depends critically on the nature of the protein substrate. The chemically inert Co(NH3)36+ ion exerts effects on protein kinase reactions similar to those of spermidine or spermine. This enhancement of the phosphorylation of various protein substrates by polyamines or Co(NH3)63+ by purified nuclear protein kinase preparations was studied in relation to effects of temperature, pH and other factors. The results provide further support for our hypothesis [Ahmed, Wilson, Goueli & Williams-Ashman (1978) Biochem. J. 176, 739-750] that the enhancement of certain protein kinase reactions by polycations relates primarily to their interaction with the protein substrate, yielding more favourable conformations for phosphorylation by the protein kinase, rather than a direct effect on its catalytic activity.This publication has 21 references indexed in Scilit:
- Polyamine-mediated protein phosphorylations: A possible target for intracellular polyamine actionMolecular and Cellular Endocrinology, 1983
- Polyamine-like effects of cobalt(III) hexaammine on various cyclic nucleotide-independent protein phosphokinase reactionsBiochemical and Biophysical Research Communications, 1983
- Fractionation and partial purification of rat liver nuclear protein kinasesInternational Journal of Biochemistry, 1982
- Occurrence of NI and NII type protein kinases in the nuclei from various tissues of the ratArchives of Biochemistry and Biophysics, 1982
- Polyamines alter the substrate preference of nuclear protein kinase NIIBiochemistry, 1982
- Two protein kinases from nuclei of cultured tobacco cells with properties similar to the cyclic nucleotide‐independent enzymes (NI and NII) from animal tissueFEBS Letters, 1982
- Cation-induced toroidal condensation of DNAJournal of Molecular Biology, 1980
- Properties of rat liver nuclear protein kinasesBiochimica et Biophysica Acta (BBA) - Enzymology, 1979
- Effects of polyamines and histone on the phosphorylation of non-histone proteins in isolated rat liver nucleiBiochemical and Biophysical Research Communications, 1978
- A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye BindingAnalytical Biochemistry, 1976