Sequence of Escherichia coli O128 antigen biosynthesis cluster and functional identification of an α‐1,2‐fucosyltransferase
- 10 September 2003
- journal article
- Published by Wiley in FEBS Letters
- Vol. 553 (1-2), 99-103
- https://doi.org/10.1016/s0014-5793(03)00980-3
Abstract
O128 is one of the most common atypical enteropathogenic Escherichia coli isolated from diarrhea patients worldwide. The primary structure of E. coli O128 repeat units has previously been determined as →3)-β-D-GalNAc-(1→4)-α-D-Gal-(1→3)-β-D-GalNAc-(1→6)-[α-L-Fuc-(1→2)]-β-D-Gal-(1→ pentasaccharide. Here we report the complete sequencing of E. coli O128 antigen biosynthesis gene cluster and its flanking regions. Comparative sequence analysis revealed the expected O128 antigen process genes, GDP-fucose biosynthesis genes and four potential glycosyltransferase genes responsible for the assembly of E. coli O128 antigen repeats. WbsJ was shown to encode an α-1,2-fucosyltransferase by enzymatic assays and nuclear magnetic resonance spectroscopy analysisKeywords
This publication has 29 references indexed in Scilit:
- Effect of O Acetylation of Neisseria meningitidis Serogroup A Capsular Polysaccharide on Development of Functional Immune ResponsesInfection and Immunity, 2002
- Lipopolysaccharide EndotoxinsAnnual Review of Biochemistry, 2002
- The O-Antigen Gene Cluster of Escherichia coli O55:H7 and Identification of a New UDP-GlcNAc C4 Epimerase GeneJournal of Bacteriology, 2002
- Synthetic Polysaccharide Type 3-Related Di-, Tri-, and Tetrasaccharide–CRM 197 Conjugates Induce Protection against Streptococcus pneumoniae Type 3 in MiceInfection and Immunity, 2001
- Lack of expression of bundle-forming pili in some clinical isolates of enteropathogenicEscherichia coli(EPEC) is due to a conserved large deletion in thebfpoperonFEMS Microbiology Letters, 1999
- Bacterial polysaccharide synthesis and gene nomenclatureTrends in Microbiology, 1996
- CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choiceNucleic Acids Research, 1994
- Examination of archetypal strains of enteropathogenic Escherichia coli for properties associated with bacterial virulenceJournal of Medical Microbiology, 1993
- Detection of an Adherence Factor of Enteropathogenic Escherichia coli with a DNA ProbeThe Journal of Infectious Diseases, 1985
- Form variation in Escherichia coli K1: determined by O-acetylation of the capsular polysaccharide.The Journal of Experimental Medicine, 1979