Binding of actinomycin D to DNA revealed by DNase I footprinting
- 28 November 1983
- journal article
- Published by Wiley in FEBS Letters
- Vol. 164 (1), 97-101
- https://doi.org/10.1016/0014-5793(83)80027-1
Abstract
We have analyzed the specificity of the actinomycin D—DNA interaction. The ‘footprint’ method has been used in this investigation. It is shown that: (i) The presence of dinucleotide GC or GG is required for binding of a single drug molecule. (ii) The strong binding sites are encoded by tetranucleotide XGCY; where X ≠ G and Y ≠ C in accordance with RNA elongation hindrance sites [1]. (iii) There is a positive cooperativity in binding of actinomycin D with DNA.This publication has 9 references indexed in Scilit:
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