RAPID, GEOGRAPHICALLY EXTENSIVE GENETIC INTROGRESSION AFTER SECONDARY CONTACT BETWEEN TWO PUPFISH SPECIES ( CYPRINODON, CYPRINODONTIDAE)

Abstract

Apparently between 1980 and 1984, Cyprinodon variegatus was introduced into an area of the Pecos River in Texas, where it hybridized with an endemic species, C. pecosensis. Protein electrophoresis indicated that, by 1985, panmictic admixtures of these two pupfishes occupied approximately 430 river-kilometers of the Pecos River, roughly one-half of the historic range of the endemic species. The average frequency of introduced alleles at four diagnostic loci ranged from 0.18 to 0.84 at the 15 sites sampled from the Pecos River in Texas. Clinal patterns in allele frequencies suggest that C. variegatus was introduced into a mid-reach of the river and that this was followed by both upstream and downstream dispersal of the introduced alleles. All pairwise combinations of loci showed significant linkage disequilibrium. The level of disequilibrium indicates chromosomal linkage for one gene-pair, Gpi-A and Est-1. The change in pupfish allele frequencies in the Pecos River represents an extreme example of rapid natural selection in a seminatural situation.