The inception of blood clotting

Abstract

It is not essential that leucocytes disintegrate for blood to begin to clot. Platelets, which had been disintegrated by means of saponin, neither produced intravascular clotting nor hastened the coagulation if collected in paraffined vessels. The lysis of blood platelets hastened the clotting of blood shed on glass and also the coagulation of blood shed into paraffined vessels when tissue juices were present. Fresh blood-serum clots both pure blood and pure blood plasma, which have been shed into paraffined vessels at once, but equivalent amounts injected intra-vascularly do not cause intravascular coagulation in non-pregnant cats. Small amounts of fresh juices of testis, muscle or kidney produced rapid coagulation of blood shed into paraffined vessels, but rapid intravascular injection of these juices did not produce clotting in vivo in non-pregnant cats. Kidney juice caused clotting in the portal vein of a pregnant cat and hastened coagulation in vitro of pure blood from a pregnant cat.