Cholesteryl esters and cholesterol were isolated from egg yolk lipids by thin-layer chromatography. The cholesteryl esters and cholesterol contents of egg yolk lipids were 0.34% and 3.64%, respectively, as determined by gas–liquid chromatography. The fatty acid composition of the cholesteryl esters was 16:0, 24.7%; 16:1, 4.6%; 18:0, 6.8%; 18:1, 53.3%; and 18:2, 10.7%.