African Trypanosomes: Cultivation of Animal-Infective Trypanosoma brucei in Vitro
- 27 May 1977
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 196 (4293), 992-994
- https://doi.org/10.1126/science.558652
Abstract
Trypanosoma brucei grew in the presence of bovine fibroblast-like cells in Hepes-buffered RPMI 1640 medium with 20 percent fetal bovine serum for more than 220 days at 37 degrees C. The organisms grown in this system were infective to mammalian hosts, retained the morphological and biochemical characteristics of long slender bloodstream forms, and displayed variant-antigen on their surfaces.This publication has 13 references indexed in Scilit:
- Identification, purification and properties of clone-specific glycoprotein antigens constituting the surface coat ofTrypanosoma bruceiParasitology, 1975
- Quantitative Ultrastructural Investigations of the Life Cycle of Trypanosoma brucei: A Morphometric Analysis*The Journal of Protozoology, 1975
- ANTIBODY‐INDUCED MOVEMENT OF PROTOZOAN SURFACE MEMBRANE ANTIGENS*Annals of the New York Academy of Sciences, 1975
- Cultivation ofTrypanosoma bruceisspp. in semi-defined and defined mediaParasitology, 1973
- Quantitative studies on trypanosomes in tsetse tissue cultureExperimental Parasitology, 1973
- Electron transport systems and mitochondrial DNA in trypanosomatidae: A reviewExperimental Parasitology, 1970
- The Fine Structure of Trypanosoma congolense in Its Bloodstream PhaseThe Journal of Protozoology, 1969
- Short Term Cultivation of Trypanosoma brucei in vitro at 37° CNature, 1967
- Polymorphism and Mitochondrial Activity In Sleeping Sickness TrypanosomesNature, 1965
- A method for the measurement of the infectivity of trypanosome suspensionsExperimental Parasitology, 1963