Ecdysteroid Metabolism by Soluble Enzymes from an Insect. Metabolic Relationships between 3β-Hydroxy-, 3α-Hydroxy- and 3-Oxoecdysteroids
- 1 January 1984
- journal article
- research article
- Published by Walter de Gruyter GmbH in Hoppe-Seyler´s Zeitschrift Für Physiologische Chemie
- Vol. 365 (2), 809-818
- https://doi.org/10.1515/bchm2.1984.365.2.809
Abstract
The formation and fate of 3-dehydroecdysteroids were studied in vitro in tissue extracts from P. brassicae (Lepidoptera). Three cytosolic enzymes are involved: an ecdysone oxidase converts 3.beta.-hydroxyecdysteroids into 3-dehydroecdysteroids and 2 different reductases, requiring NADPH as cofactor, transform 3-dehydroecdysteroids into the 3.alpha.-hydroxy- or the 3.beta.-hydroxy forms, respectively. 3.alpha.-hydroxyecdysteroid formation is only detected in the gut; all tissues contain the 2 other activities. Conversion of 3.beta.- to 3.alpha.-hydroxyecdysteroids apparently proceeds through 3-dehydro compounds. The biological significance of these reactions is discussed.This publication has 15 references indexed in Scilit:
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