Coated vesicles from human placenta carry ferritin, transferrin, and immunoglobulin G.

Abstract

Coated vesicles prepared by using isotonic gradients retain their contents. Ferritin, transferritin and IgG were detected in human placental coated vesicles. Triton X-100 extraction of crude coated vesicles removes contaminating uncoated membranes, leaving coated particles and their associated proteins apparently intact; this provides an efficient means of preparing large quantities of coated vesicle proteins. The clathrin cages surround cores of structural protein containing polypeptides of 100,000 and 50,000 MW.