Kinetics of RNA Labeling in Toad Bladder Epithelium: Effects of Aldosterone and Related Steroids
- 1 August 1974
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 71 (8), 3101-3105
- https://doi.org/10.1073/pnas.71.8.3101
Abstract
Sucrose gradient analysis revealed an aldosterone-dependent increase in the incorporation of [(3)H]uridine into a nonmethylated, rapidly labeled RNA that sedimented at 9-12 S. This effect was antagonized by an anti-mineralocorticoid, spironolactone (SC 9420), and was not elicited either by a glucocorticoid, cortisol, or by the inactive isomer, 17-alpha-isoaldosterone. These findings are in accord with the inference that mRNA is induced by aldosterone during the latent period and that the induced mRNA mediates the action on sodium transport.Keywords
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