Kinetics of RNA Labeling in Toad Bladder Epithelium: Effects of Aldosterone and Related Steroids
- 1 August 1974
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 71 (8), 3101-3105
- https://doi.org/10.1073/pnas.71.8.3101
Abstract
Sucrose gradient analysis revealed an aldosterone-dependent increase in the incorporation of [(3)H]uridine into a nonmethylated, rapidly labeled RNA that sedimented at 9-12 S. This effect was antagonized by an anti-mineralocorticoid, spironolactone (SC 9420), and was not elicited either by a glucocorticoid, cortisol, or by the inactive isomer, 17-alpha-isoaldosterone. These findings are in accord with the inference that mRNA is induced by aldosterone during the latent period and that the induced mRNA mediates the action on sodium transport.Keywords
This publication has 23 references indexed in Scilit:
- Modification of Rat Renal Cortical Chromatin by Aldosterone TreatmentCanadian Journal of Biochemistry, 1971
- Kinetics of RNA synthesis in toad bladder epithelium: action of aldosterone during the latent periodJournal of Clinical Investigation, 1971
- Effects of sodium transport stimulating substances on enzyme activities in the toad bladderPflügers Archiv - European Journal of Physiology, 1970
- Reversible stimulation of sodium transport in the toad bladder by stretchJournal of Clinical Investigation, 1969
- Studies on Renal Aldosterone-binding ProteinsJournal of Biological Chemistry, 1968
- Induction of RNA and protein synthesis in the action of aldosterone in the ratAmerican Journal of Physiology-Legacy Content, 1967
- Characteristics of the renal nuclear receptors for aldosterone.Proceedings of the National Academy of Sciences, 1966
- On the mechanism of action of aldosterone on sodium transport: effects of inhibitors of RNA and of protein synthesis.1966
- RNA metabolism in the HeLa cell nucleusJournal of Molecular Biology, 1966
- The Determination of Nucleic AcidsPublished by Wiley ,1966