A head inhibitory gradient arising in the vicinity of a hydra's head, and a foot inhibitory gradient arising from the animal's foot are thought to intersect in the budding region. To investigate the possible influences of these gradients on budding, samples were prepared of 25 or more animals having as many as five gastric regions or gastric-plus-budding regions grafted in tandem. The segments of grafted animals were considered equivalent except for distance from the head and foot. The average frequencies of regeneration of budding regions on grafted gastric regions, and the average number of buds on grafted budding regions were determined for each sample.