Critical Evaluation of Feulgen Microspectrophotometry for Estimating Amount of DNA in Cell Nuclei
- 17 December 1954
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 120 (3129), 1022-1023
- https://doi.org/10.1126/science.120.3129.1022
Abstract
A comparison of the DNA content measured in arbitrary units from a large variety of animal nuclei was made using biochemical analysis and ultra violet as well as Feulgen microspectrophotometry. The Feulgen test can be a reliable tool for the relative amounts of DNA provided the differences to be detected are sufficiently large; small differences must be interpreted with caution.Keywords
This publication has 4 references indexed in Scilit:
- Quantitative Aspects of Nuclear NucleoproteinsInternational Review of Cytology, 1953
- Unreliability of the data hitherto reported on the desoxypentosenucleic acid content of cell nuclei determined by microspectrophotometryBiochimica et Biophysica Acta, 1952
- A Critical Evaluation of Quantitative Histo- and Cytochemical Microscopic TechniquesScience, 1951
- NUCLEOPROTEIN DETERMINATION IN CYTOLOGICAL PREPARATIONSPublished by Cold Spring Harbor Laboratory ,1947