Pax3/Pax7 mark a novel population of primitive myogenic cells during development

Abstract

Skeletal muscle serves as a paradigm for the acquisition of cell fate, yet the relationship between primitive cell populations and emerging myoblasts has remained elusive. We identify a novel population of resident Pax3⁺/Pax7⁺, muscle marker-negative cells throughout development. Using mouse mutants that uncouple myogenic progression, we show that these Pax⁺ cells give rise to muscle progenitors. In the absence of skeletal muscle, they apoptose after down-regulation of Pax7. Furthermore, they mark the emergence of satellite cells during fetal development, and do not require Pax3 function. These findings identify critical cell populations during lineage restriction, and provide a framework for defining myogenic cell states for therapeutic studies.