Benzo(a)pyrene metabolites formed by the action of yeast cytochrome P‐450/P‐448
- 1 January 1979
- journal article
- research article
- Published by Wiley in Journal of Chemical Technology and Biotechnology
- Vol. 29 (5), 320-324
- https://doi.org/10.1002/jctb.503290508
Abstract
Benzo(a)pyrene metabolites have been identified by high‐pressure liquid chromatography after production by a cytochrome P‐450 in brewer's yeast disruptates. The major metabolites are 7,8‐dihydro‐7,8‐dihydroxy‐benzo(a)pyrene, 9‐hydroxybenzo(a)‐pyrene and 3‐hydroxybenzo(a)pyrene. Pretreatment of the yeast with benzo(a)‐pyrene, during its growth decreases the Michaelis constant (Km) of the enzyme for benzo(a)pyrene, using either NADPH or cumene hydroperoxide as a cofactor.This publication has 12 references indexed in Scilit:
- Partial Repression of Cytochrome P-450 by Adenosine 3′:5′-Cyclic Monophosphate in Protoplasts of Saccharomyces cerevisiaeBiochemical Society Transactions, 1978
- Rapid and Economical Production of Microsomal Cytochrome P-450 in Yeast Resuspended in 20% Glucose Medium: Relationship to the Biosynthesis of Mitochondrial CytochromesBiochemical Society Transactions, 1977
- The metabolism of benzo(a)pyrene, 7,8-dihydro-7,8-dihydroxybenzo(a)pyrene and 9,10-dihydro-9,10-dihydroxybenzo(a)pyrene by short-term organ cultures of hamster lungBiochemical Pharmacology, 1977
- Relationship of Cytochrome P-450 to Growth Phase of Brewer's Yeast in 1%- or 20%-Glucose MediumBiochemical Society Transactions, 1975
- Metabolic activation of benzo(a)pyrene proceeds by a diol-epoxideNature, 1974
- Epoxides in Polycyclic Aromatic Hydrocarbon Metabolism and CarcinogenesisAdvances in Cancer Research, 1974
- A modified method for the assay of benzo[a]pyrene hydroxylaseAnalytical Biochemistry, 1973
- A Comparative study on the effects of phenobarbital and 3,4-benzpyrene on the hydroxylating enzyme system of rat-liver microsomesChemico-Biological Interactions, 1969
- Metabolism of 3,4-BenzopyreneScience, 1955
- The elimination of 3:4-benzpyrene from the animal body after subcutaneous injectionBiochemical Journal, 1941