Application of 125I radioimmunoassay to measure inhibition of precipitin reactions using carbohydrate-specific antibodies

Abstract

Antibodies raised [in rabbits] to an artificial antigen with .beta.-D-galactopyranosyl groups as antigenic determinants were purified using an immunoadsorbent prepared from the hapten involved in the synthesis of the antigen. To study of the specificity of these antibodies, 125I radiolabeling of the artificial antigen or the antibody was used in the study of inhibitions of the precipitin reaction. The method, involving labeling of the artificial antigen and counting radioactivity in the supernatant, was more accurate and faster than the usual methods based on measuring the amount of protein precipitated by chemical or spectroscopic methods.