Phosphorylation of high-molecular-weight membrane protein species in Chinese-hamster ovary cells in culture: effect of 6-N,2′-O-dibutyryladenosine 3′:5′-cyclic monophosphate plus testosterone (Short Communication)
- 15 March 1973
- journal article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 132 (3), 641-644
- https://doi.org/10.1042/bj1320641
Abstract
Growth of Chinese-hamster ovary cells in [32P]phosphate and [3H]leucine and subsequent assay of the plasma membranes reveals phosphorylation in two protein regions corresponding to molecular weights of 280000 and 195000. Culture in the presence of the 6-N,2-O′-dibutyryl derivative of cyclic AMP plus testosterone does not stimulate [32P]-phosphate incorporation, but determines a modification in the qualitative pattern of phosphorylation.Keywords
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