Expression in normal adult, fetal, and neoplastic tissues of a carbohydrate differentiation antigen recognised by antigranulocyte mouse monoclonal antibodies.
Open Access
- 1 May 1985
- journal article
- research article
- Published by BMJ in Journal of Clinical Pathology
- Vol. 38 (5), 521-529
- https://doi.org/10.1136/jcp.38.5.521
Abstract
The distribution in paraffin fixed human tissues of a carbohydrate antigen defined by two monoclonal antibodies raised against human granulocytes has been studied by means of an immunoperoxidase technique. In addition to granulocytes, the antigen has been detected in adult tissues on identifiable cell types of the stomach, kidney, adrenal medulla, and brain and on the mucins of the gastrointestinal tract and other secretions. In fetal tissue, epithelial cells of the alimentary tract, lung, brain, and kidney express the antigen. Adenocarcinoma of the colon, stomach, breast, and lung are stained strongly, as are other types of lung cancer. The monoclonal antibodies give a staining pattern similar but not identical to other monoclonal antibodies raised against granulocytes or neoplastic cell lines which recognise the antigen 3-fucosyl N-acetyllactosamine. The use of antibodies against this oncofetal antigen in the study of differentiation and as tumour markers is discussed.Keywords
This publication has 25 references indexed in Scilit:
- Widespread distribution in human tissues of an antigenic determinant of granulocytes.Journal of Clinical Pathology, 1984
- Many monoclonal antibodies with an apparent specificity for certain lung cancers are directed against a sugar sequence found in lacto-N-fucopentaose IIIArchives of Biochemistry and Biophysics, 1983
- Marker of peripheral blood granulocytes and monocytes of man recognized by two monoclonal antibodies VEP8 and VEP9 involves the trisaccharide 3‐fucosyl‐N‐acetyllactosamineEuropean Journal of Immunology, 1982
- Monoclonal antibodies directed against the sugar sequence of lacto-N-fucopentaose III are obtained from mice immunized with human tumorsArchives of Biochemistry and Biophysics, 1982
- Neutrophil granulocytic cell antigen defined by a monoclonal antibody--its distribution within normal haemic and non-haemic tissue.Journal of Clinical Pathology, 1982
- The monoclonal antibody anti‐SSEA‐1 discriminates between fucosylated type 1 and type 2 blood group chainsFEBS Letters, 1981
- Stage-specific embryonic antigen involves αl→ 3 fucosylated type 2 blood group chainsNature, 1981
- The Preparation and Properties of Monoclonal Antibodies against Human Granulocyte Membrane AntigensBritish Journal of Haematology, 1981
- Demonstration of carcinoembryonic antigen in human breast carcinomas by the immunoperoxidase technique.Journal of Clinical Pathology, 1980
- A new antigen on the epithelial membrane: its immunoperoxidase localisation in normal and neoplastic tissue.Journal of Clinical Pathology, 1979