The fluorescence decay of aqueous tryptophan is shown to be described by a two exponential decay function whose components have life-times of 3.14 ns and 0.51 ns. These components are assigned as the solvent equilibrated 1La and 1Lb, states respectively. The time-resolved emission spectra are presented and can be resolved into two spectra with λmax at 350 nm and 335 nm corresponding to these two states.