Conformation and movement of the vegetative nucleus of the angiosperm pollen tube: association with the actin cytoskeleton
Open Access
- 1 June 1989
- journal article
- Published by The Company of Biologists in Journal of Cell Science
- Vol. 93 (2), 299-308
- https://doi.org/10.1242/jcs.93.2.299
Abstract
Actin is present in the cytoplasm of the vegetative cell of angiosperm pollens in numerous fusiform, spiculate or toroidal bodies, and also as a sheath enveloping the vegetative nucleus. During activation following hydration, the compact cytoplasmic bodies are translated into skeins of extended fibrils, and circulatory movements begin in the cytoplasm. Throughout this period the vegetative nucleus, with fibrillar actin now associated with the surface, undergoes a continuous change of shape. In the extending tube following germination the actin cytoskeleton consists of numerous mainly longitudinally oriented fibrils. After entry into the tube the vegetative nucleus remains associated with the fibrils, usually extending greatly in length and developing attenuated, often pointed extensions. The observed conformations, which change continuously, suggest that varying local tensions are applied to the vegetative nucleus during passage through the tube. Cytochalasin D breaks up the actin fibril system and brings about a rapid contraction of the nucleus, at the same time eliminating the elastic extensions of the nuclear envelope. Nuclei isolated physically from unfixed tubes also contract in length as the fibrillar components of the cytoskeleton are detached. These findings indicate that the movement of the vegetative nucleus depends on local associations of the nuclear envelope with the actin cytoskeleton of the vegetative cell.Keywords
This publication has 13 references indexed in Scilit:
- Organization of the cytoskeleton in pollen tubes ofPyrus communis: a study employing conventional and freeze-substitution electron microscopy, immunofluorescence, and rhodamine-phalloidinProtoplasma, 1988
- Spatial and temporal organization of actin during hydration, activation, and germination of pollen inPyrus communis L.: a population studyProtoplasma, 1988
- An actin network is present in the cytoplasm throughout the cell cycle of carrot cells and associates with the dividing nucleus.The Journal of cell biology, 1987
- Ultrastructure of the cytoskeleton in freeze-substituted pollen tubes ofNicotiana alataProtoplasma, 1987
- Fluorescence staining of the actin cytoskeleton in living cells with 7-nitrobenz-2-oxa-1,3-diazole-phallacidin.Proceedings of the National Academy of Sciences, 1980
- The identification of F actin in the pollen tube and protoplast of Amaryllis belladonnaExperimental Cell Research, 1974
- Tubular and filamentous structures in pollen tubes: Possible involvement as guide elements in protoplasmic streaming and vectorial migration of secretory vesiclesPlanta, 1972
- A Phase-Contrast Microscopic Study of Pollengrain Germination, Nuclear Movement and Pollentube Mitosis in Tradescantia virginianaCYTOLOGIA, 1962
- The male gametophyte of angiospermsThe Botanical Review, 1949
- Zytologische Beobachtungen über die Entwicklung der Pollenschläuche einiger AngiospermenPlanta, 1930