Identification and nucleotide sequence of a diversity DNA segment (D) of immunoglobulin heavy-chain genes
- 1 April 1981
- journal article
- research article
- Published by Springer Nature in Nature
- Vol. 290 (5807), 562-565
- https://doi.org/10.1038/290562a0
Abstract
A putative diversity segment of Ig heavy-chain genes (D segment) was identified 700 base pairs 5'' to JH1 DNA on the germ-line genome of the mouse. This 10-base pair D segment is flanked by 2 sets of sequences which are possible recognition sites for a recombinase. The spacer separating the heptamer and the nonamer is 12 base pairs long on both sides of the D segment. As the spacer separating the 2 signal sequences in VH DNA and JH DNA is 23 .+-. 1 base pairs long, the 2 recombinations required for creation of a complete Ig VH gene, a VH-D joining and a D-JH joining, follow a 12/23-base pair spacer rule. Allelic exclusion is discussed with respect to D segments.Keywords
This publication has 42 references indexed in Scilit:
- Exon shuffling generates an immunoglobulin heavy chain gene.Proceedings of the National Academy of Sciences, 1980
- A κ-immunoglobulin gene is formed by site-specific recombination without further somatic mutationNature, 1979
- Sequences of five potential recombination sites encoded close to an immunoglobulin kappa constant region gene.Proceedings of the National Academy of Sciences, 1979
- Sequences at the somatic recombination sites of immunoglobulin light-chain genesNature, 1979
- Sequences of mouse immunoglobulin light chain genes before and after somatic changesCell, 1978
- DNA clones containing mouse immunoglobulin kappa chain genes isolated by in vitro packaging into phage lambda coats.Proceedings of the National Academy of Sciences, 1978
- A complete immunoglobulin gene is created by somatic recombinationCell, 1978
- Multiple related immunoglobulin variable-region genes identified by cloning and sequence analysis.Proceedings of the National Academy of Sciences, 1978
- Variable and constant parts of the immunoglobulin light chain gene of a mouse myeloma cell are 1250 nontranslated bases apart.Proceedings of the National Academy of Sciences, 1977
- Evidence for somatic rearrangement of immunoglobulin genes coding for variable and constant regions.Proceedings of the National Academy of Sciences, 1976