Temperature Relations in Phagocytosis

Abstract

In a series of studies of phagocytosis in vitro, exudative guinea pig and rabbit polymorphonuclear leukocytes in a system with fresh homologous normal serum and Staphylo-coccus aureus showed enhanced phagocytic powers with 5[degree]C increments within a temp. range of 22[degree] and 42[degree]C. These results are based on 10-min. incubation of the complete phagecytic system in a controlled water bath. Data here included are from the 15 tests (10 guinea pig and 5 rabbit) of which the techniques were the most satisfactorily standardized. In all, over 30 expts. were performed, and, regardless of variations in technique, the reaction showed the same trend. 15 expts. with mice did not lead to clear-cut results; this was due to failure to secure an adequate leuko-cytic response. Results of the 5 tests in which there was a moderate amt. of exudation are presented; they indicate an increased ingestion up to 37[degree]C followed by a decline at 42[degree]C. In 5 tests with guinea pig leukocytes and fresh homologous normal serum (vs. Staphylococcus aureus) in which the temps. ranged from 37[degree] to 45[degree]C with 2[degree]C increments, and with comparisons at 25[degree] and 50 [degree]C, phagocytosis increased to a point approximating 43[degree]C and declined rapidly beyond that point. The increase in phagocytosis proceeded at a slightly decreased rate as elevated temps. were reached.