Since my former report1there has been a prodigious amount of work done covering many phases of vitamin B. The chemical and biologic properties have received the greatest attention, as evidenced by the many reports of research that have been published. The problem seems to be world wide if one takes into account the geographic sources of these studies. The greater part of the work is still being done on animals, particularly on birds, a group that is most susceptible to this deficiency disease. The chemists also have been exceedingly busy in this field. I shall give a summary of the most recent observations of biologists and biochemists before proceeding to the original work with which this paper deals. The most striking development in the studies of vitamin B is the discovery that there are several different factors in what was originally considered as vitamin B. Goldberger2has