Fixation for immunofluorescence and enzyme histochemistry on serial sections.

Abstract

A technique of tissue preparation is described that allows lysosomal and other enzymes to be used as cell markers in conjunction with cell surface and cytoplasmic antigens. By using serial sections, a phenotypic characterization of individual cell types in situ in the lesion can be built up, reducing some of the handicaps of antiserum specificity and leading to a greater understanding of the pathogenesis of the disease process. This technique was used for the study of human and mouse lesions.