Restoration of X-ray Resistance and V(D)J Recombination in Mutant Cells by Ku cDNA
- 14 October 1994
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 266 (5183), 288-291
- https://doi.org/10.1126/science.7939667
Abstract
Three genetic complementation groups of rodent cells are defective for both repair of x-ray-induced double-strand breaks and V(D)J recombination. Cells from one group lack a DNA end-binding activity that is biochemically and antigenically similar to the Ku autoantigen. Transfection of complementary DNA (cDNA) that encoded the 86-kilodalton subunit of Ku rescued these mutant cells for DNA end-binding activity, x-ray resistance, and V(D)J recombination activity. These results establish a role for Ku in DNA repair and recombination. Furthermore, as a component of a DNA-dependent protein kinase, Ku may initiate a signaling pathway induced by DNA damage.Keywords
This publication has 19 references indexed in Scilit:
- Ku80: product of the XRCC5 gene and its role in DNA repair and V(D)J recombinationScience, 1994
- Regional Assignment of a Human DNA Repair Gene (XRCC5) to 2q35 by X-Ray Hybrid MappingGenomics, 1994
- Chromosomal location and expression of the genes coding for Ku p70 and p80 in human cell lines and normal tissuesCytogenetic and Genome Research, 1994
- DNA damage and the DNA-activated protein kinaseTrends in Biochemical Sciences, 1993
- Subchromosomal localization of a gene (XRCC5) involved in double strand break repair to the region 2q34-36Somatic Cell and Molecular Genetics, 1993
- Impairment of V(D)J Recombination in Double-Strand Break Repair MutantsScience, 1993
- Mutant rodent cell lines sensitive to ultraviolet light, ionizing radiation and cross-linking agents: a comprehensive survey of genetic and biochemical characteristicsMutation Research/DNA Repair, 1993
- Homologous recombination in a Chinese hamster X-ray-sensitive mutantMutation Research, 1986
- Characterization of a high molecular weight acidic nuclear protein recognized by autoantibodies in sera from patients with polymyositis-scleroderma overlap.Journal of Clinical Investigation, 1981
- A new technique for the assay of infectivity of human adenovirus 5 DNAVirology, 1973