Chromosomal Location of the mop (groE) Gene Necessary for Bacteriophage Morphogenesis in Escherichia coli

Abstract

Summary: The chromosomal location of a host gene, mop (groE), which is essential for the morphogenesis of several bacteriophages in Escherichia coli, was determined by two- and three-factor transductional crosses using phage P1. Cotransduction frequencies between mop and other markers were: asp A, 90%; amp A, 77%; frdA, 73%; mel, 24%. The sequence of markers in the corresponding segment (mel to purA; 91·5 to 93·5 min) of the E. coli linkage map was shown to be mel-aspA-mop(groE)-ampA-frdA-purA.