Histocompatibility antigens on astrocytoma cells.
Open Access
- 1 March 1982
- journal article
- research article
- Published by BMJ in Journal of Neurology, Neurosurgery & Psychiatry
- Vol. 45 (3), 193-198
- https://doi.org/10.1136/jnnp.45.3.193
Abstract
Biopsies tumour cells from astrocytoma-bearing patients were grown in primary culture for 3-5 days. Both low and high grade tumours were represented in the study. The cultured cells could be shown to express the HLA-A and -B antigens using a multispecific allo-antiserum and a rabbit anti-beta-2 microglobulin antibody. The tumour cells were negative for the HLA-DR determinants when tested with either rabbit anti-Ia-like antisera or specific anti-HLA-DR allo-antisera. They also failed to stimulate allogeneic lymphocytes in primary mixed lymphocyte-tumour cell cultures but stimulated lymphocytes primed to tumour cells in vitro. The tumour cells were also capable of stimulating autologous lymphocytes from the tumour-bearing patient in most of the combinations tested.This publication has 16 references indexed in Scilit:
- Cytotoxic antibody responses in astrocytoma patientsJournal of Neurosurgery, 1979
- Human tumor-lymphocyte interactionin vitro. III. T lymphocytes in autologous tumor stimulation (ATS)International Journal of Cancer, 1978
- Unique Aspects of Central Nervous System ImmunologyNeurosurgery, 1978
- Detection of Thrombocyte Antibodies by125I Labeled Protein ATissue Antigens, 1978
- Cell mediated lympholysis: CML. A microplate technique requiring few target cells and employing a new method of supernatant collectionJournal of Immunological Methods, 1977
- A microassay for antibody binding to tumor cell surface antigens using 125I-labelled protein a from Staphylococcus aureusJournal of Immunological Methods, 1977
- Two Different VH Gene Products Make Up the T‐Cell ReceptorsScandinavian Journal of Immunology, 1976
- HL‐A and Disease Associations ‐ A SurveyImmunological Reviews, 1975
- Tumor-specific membrane antigens in established cell lines from gliomasCancer, 1974
- Surface antigen(s) common to human astrocytoma cellsNature, 1974