Molecular cloning and regulation of expression of the genes for initiation factor 3 and two aminoacyl-tRNA synthetases
- 1 October 1982
- journal article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 152 (1)
- https://doi.org/10.1128/jb.152.1.357-362.1982
Abstract
A 22-kilobase fragment of the Escherichia coli chromosome which contains the genes for translation initiation factor 3, phenylalanyl-tRNA synthetase, and threonyl-tRNA synthetase was cloned into plasmid pACYC184. The hybrid plasmid (designated pID1) complements a temperature-sensitive pheS lesion in E. coli NP37. pID1-transformed NP37 overproduce initiation factor 3 and phenylalanyl-tRNA synthetase. Gene expression from pID1 was studied in vitro in a coupled transcription-translation system and in minicells. The results suggest that the genes for initiation factor 3 and phenylalanyl- and threonyl-tRNA synthetase are regulated by different mechanisms.Keywords
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